Recombinant Human NT-4

Name

Recombinant Human NT-4 (Recombinant Human Neurotrophin-4; rHuNT-4)；重组人神经营养因子4

Synonyms

NT-5; NTF4; NTF5; GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 4; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; Neurotrophin-5; neurotrophin-5; Neutrophic factor 4; NT4; NT-4; NT-4/5; NT-5; NTF5NT5

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50ng/ml, corresponding to a specific activity of >2.0×10⁴IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH5.5.

Endotoxin

Less than 1EU/μg of rHuNT-4 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

NT-4 also named as NT-5 is a neuronal and epithelial grow factor belongs to thengF-beta family. The NT-4 precursor is consisted of a 24a.a. signal peptide, a 56a.a. propertied and 130a.a. NT-4. The mature protein has six Cys amino acid residues and has the relative structure with NT-3, BDNF (sharing about 48%-52% sequence identity). Additionally, it shares 91% and 95%a.a. sequence identity with mouse and rat NT-4. NT-4 is mainly expressed in prostate and has low level thymus, placenta, and skeletal muscle. It can binding with the LNGFR and trkB receptors and plays a crucial role in the regulation of survival and the maintenance of peripheral sensory sympathetic neurons. Defect of NT-4 may cause primary open angle glaucoma type 1O.

Amino Acid Sequence

MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

产品编号

产品名称

包装

KL-5678-2μg

Recombinant Human NT-4

2μg

KL--5678-10μg

Recombinant Human NT-4

10μg

KL-5678-100μg

Recombinant Human NT-4

100μg

KL-5678-1mg

Recombinant Human NT-4

1mg

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说明书

1份