Recombinant Human NRG1-α (Recombinant Human Neuregulin 1-α EGF Domain; rHuNRG1-α);重组人神经调节蛋白1-α
Synonyms
ARIA; GGF2; HGL; HRG; HRG1; MST131; MSTP131; neuregulin 1; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40ng/ml, corresponding to a specific activity of >2.5×104IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in 20mM PB, pH6.0, 150mM NaCl.
Endotoxin
Less than 0.1EU/μg of rHuNRG1-α as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Neuregulin 1 (NRG1) is in humans encoded by the NRG1 gene. It is one of four proteins belonging to the neuregulin family that act on the EGFR family of receptor. Neuregulin 1 is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. All NRG1 isoforms contain an EGF like domain (α or β splice variant that differ in their C terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. NRG1 is necessary for cardiac development, structural maintenance, and functional integrity of the heart. NRG1 and its receptor family ErbB can play a beneficial role in the treatment of chronic heart failure (CHF) by promoting survival of cardiac myocytes, improving sarcomeric structure, balancing Ca2+homeostasis, and enhancing pumping function.