Recombinant Human BMP-2

Name

Recombinant Human BMP-2 (Recombinant Human Bone Morphogenetic Protein 2; rHuBMP-2)；重组人骨形态发生蛋白2

Synonyms

BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200ng/ml, corresponding to a specific activity of >5.0×10³IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution containing 10mM sodium citrate pH3.5.

Endotoxin

Less than 1EU/μg of rHuBMP-2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H₂O to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in PBS or cell culture media.

Category

Cytokine

Background

Bone Morphogenetic Protein 2 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-2 is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. It is a potent osteoinductive cytokine and plays its role in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. As it shown to stimulate the production of bone, the recombinant human BMP-2 is available for orthopaedic usage and dentisty. The functional form of BMP-2 is a disulflde-linked homodimer and about 26kDa The precursor of BMP-2 is 259a.a., containing the 23a.a. Nterminal signal sequence which will be cleaved by a furin-type protease to form the mature peptide.

Amino Acid Sequence

MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

产品编号

产品名称

包装

KL-5754-2μg

Recombinant Human BMP-2

2μg

KL-5754-10μg

Recombinant Human BMP-2

10μg

KL-5754-100μg

Recombinant Human BMP-2

100μg

KL-5754-1mg

Recombinant Human BMP-2

1mg

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说明书

1份