Recombinant Human EMAP-II

Name

Recombinant Human EMAP-II (Recombinant Human Endothelial-Monocyte Activating Polypeptide II; rHuEMAP-II)；重组人内皮单核细胞活化多肽II

Synonyms

AIMP1; SCYE1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; EMAP-2; EMAPIImember 1 (endothelialmonocyte-activating); endothelial-monocyte activating polypeptide II; Multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40ng/ml, corresponding to a specific activity of >2.5×10⁴IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuEMAP-II as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Endothelial-Monocyte Activating Polypeptide II (EMAP-II) is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues.

Amino Acid Sequence

SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK

产品编号

产品名称

包装

KL--5381-5μg

Recombinant Human EMAP-II

5μg

KL-5381-20μg

Recombinant Human EMAP-II

20μg

KL-5381-100μg

Recombinant Human EMAP-II

100μg

KL--5381-1mg

Recombinant Human EMAP-II

1mg

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说明书

1份