Recombinant Human IL-33

Name

Recombinant Human IL-33 (Recombinant Human Interleukin-33; rHuIL-33)；重组人白细胞介素33

Synonyms

C9orf26; DVS27; NF-HEV; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, 150mM NaCl, 1mM EDTA, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-33 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20%a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. It elicits its biological effects by interacting with IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58%a.a. sequence identity with mouse and rat IL-33.

Amino Acid Sequence

SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET

产品编号

产品名称

包装

KL-5237-2μg

Recombinant Human IL-33

2μg

KL--5237-10μg

Recombinant Human IL-33

10μg

KL-5237-100μg

Recombinant Human IL-33

100μg

KL-5237-1mg

Recombinant Human IL-33

1mg

-

说明书

1份