Recombinant Human IL-36α, 153a.a. (Recombinant Human Interleukin-36α, 153a.a.; rHuIL-36α, 153a.a.);重组人白细胞介素36α,153a.a.
Synonyms
IL36A; FIL1E; IL1E; IL1F6
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10ng/ml, corresponding to a specific activity of >1.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, 300mM NaCl, pH8.0, 0.1% Tween 80.
Endotoxin
Less than 1EU/μg of rHuIL-36α, 153a.a. as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T cells. Recombinant human interleukin-36 alpha (153a.a.) contains 153 amino acids residues which is a single non-glycosylated polypeptide and it shares 57-68%a.a. sequence identity with mouse, rabbit, equine and bovine IL-36α and 27-57%a.a. sequence identity with other novel IL-1 family members.