HSP70 Antibody

品牌:CST，产品名称：HSP70 Antibody #4872
英文名称：HSP70 Antibody
Product name：HSP70 Antibody #4872
Isotype：Mouse IgG1
Sensitivity：Endogenous
MW(kDa)：70
Applications：WB , IHC ,
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSP70. Antibodies are purified by protein A and peptide affinity chromatography.
Background： HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). Both HSP70 and HSP90 are able to interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP- and co-chaperone-dependent manner (1). HSP70 has a broad range of substrates including newly synthesized and denatured proteins, while HSP90 tends to have a more limited subset of substrates, most of which are signaling molecules. HSP70 and HSP90 often function collaboratively in a multi-chaperone system, which requires a minimal set of co-chaperones: HSP40, Hop, and p23 (2,3). The co-chaperones either regulate the intrinsic ATPase activity of the chaperones or recruit chaperones to specific substrates or subcellular compartments (1,4). When the ubiquitin ligase CHIP associates with the HSP70/HSP90 complex as a cofactor, the unfolded substrates are subjected to degradation by the proteasome (4). The biological functions of HSP70/HSP90 extend beyond their chaperone activity. They are essential for the maturation and inactivation of nuclear hormones and other signaling molecules (1,3). They also play a role in vesicle formation and protein trafficking (2).

Entrez-Gene Id

3303

Swiss-Prot Acc.

P0DMV8

Specificity / Sensitivity:

HSP70 Antibody detects endogenous levels of total HSP70 protein (HSP70-Hom, HSP70-1) and HSPC70.

Species Reactivity:

Human, Mouse, Rat, Monkey, Bovine

Application	Dilutions
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:200

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.