Product Information：
| Product name | NLRP3 Polyclonal Antibody |
| Isotype | IgG |
| Form | Lyophilized or Liquid |
| Tested applications | WB,IF,ELISA |
| Species reactivity | Human,Mouse,Rat |
| Host Species | Rabbit |
| Purity | Affinity purification |
Immunogen Information：
| Immunogen | Recombinant protein of human NLRP3 |
| Calculated molecular weight | 83kDa/105kDa/111kDa/112kDa/115kDa/118kDa |
| Observed molecular weight | 118kDa |
| Swiss Prot | Q96P20 |
| Gene ID (NCBI) | 114548 |
| Alternative Names | NLRP3; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1; NLR family pyrin domain containing 3 |
Application Notes：
| WB | 1:200 - 1:1000 |
| IF | 1:50 - 1:200 |
| | |
| Western blot analysis of extracts of various cell lines, using NLRP3 antibody. | | Immunofluorescence analysis of mouse lung using NLRP3 antibody. |
Background：
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
Storage：
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
