BrdU Antibody

BrdU Antibody

CATALOGUE NUMBER

ANT-049

TYPE

Mouse Antibody Monoclonal.

CLONE

YR-BrdU.

IMMUNOGEN

5-iodouridine covalently coupled to KLH.

IG SUBCLASS

mouse IgG1

PURIFICATION METHOD

Protein A column

FORMULATION

1mg/ml in PBS (after reconstitution).

SHIPPING CONDITIONS

Antibody is shipped lyophilized at ambient temperature.

STORAGE PROCEDURES

In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, 
if not intended for use within a month, aliquot and store at -20°C.

SOLUBILITY

Reconstitute with H₂0. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

APPLICATIONS

Immunohistochemistry, flow cytometry.

TITER

1:500- 1:2,000 (assay dependent)

NOTE

This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.