Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Cyclin A2 protein by 2.5 μg of Cyclin A2 antibody (GTX103042) 10% SDS-PAGE The immunoprecipitated Cyclin A2 protein was detected by Cyclin A2 antibody (GTX103042) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cyclin A2 antibody detects CCNA2 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10% SDS-PAGE
Cyclin A2 antibody (GTX103042) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A (GTX103042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Cyclin A2 antibody detects Cyclin A2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin A2 stained by Cyclin A2 antibody (GTX103042) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody (GTX103042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.