Human 2,3-Disphosphoglycerate (2,3-DPG) ELISA Kit

简写：2,3DPG
别名：N/A
检测范围：0.312-20 μmol/mL
产品概述：
特异性：This assay has high sensitivity and excellent specificity for detection of Human 2,3DPG. No significant cross-reactivity or interference between Human 2,3DPG and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Human 2,3DPG and the recovery rates were calculated by comparing the measured value to the expected amount of Human 2,3DPG in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human 2,3DPG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate 2,3DPG in samples. An antibody specific for 2,3DPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any2,3DPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 2,3DPG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 2,3DPG bound in the initial step. The color development is stopped and the intensity of the color is measured.