Recombinant Human Trypsi

DESCRIPTIONS

Trypsin is a member of the serine protease family. Trypsin will cleave peptides on the C-terminal side of lysine and arginine amino acid residues. Recombinant human Trypsin is a genetically engineered protein, expressed in E.Coli and purified by high pressure liquid chromatography. The amino acid sequence is the same as that of human trypsin 2. The pH optimum of trypsin is pH7 - 9. The enzyme is inhibited by serine proteases inhibitors, e.g. PMSF, and metal chelating agents, e.g., EDTA. There is no foreign enzyme activity such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF contain in preparation

SALIENT ADVANTAGES

●Animal origin free

Recombinant Human Trypsin eliminates the risk of viruses, or other potential adventitious agents found in animal derived trypsin. Which belongs to the AOF level 3 products.

●Recombinant human trypsin

The amino acid sequence is the same as that of human trypsin 2.

●Stable

A sterile recombinant trpsin lyophilized powder eliminates the contamination opportunity and

decreases the chance of activity loss in the process of transport and storage.

●High purity

1)Recombinant human trypsin provides increased specificity and eliminates contaminating activities found in lower purity enzymes

2) No other proteases contaminated such as chymotrypsin,carboxypeptidase A.

3)One single main band at 24kD by SDS-PAGE

SALIENT FEATURES

PRODUCTS INFORMATION

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute

in a reaction volume of 3.0ml at pH7.6 at 25℃, with BAEE as a substrate

(1cm light path).

RELATIVE PRODUCTS: Recombinant Porcine Trypsin,

Recombinant Trypsin Cell Disassociated Solution