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DNA Damage Antibody Sampler Kit

DNA Damage Antibody Sampler Kit
5950 1EA 起订
上海 更新日期:2020-01-13

上海恒斐生物科技有限公司

非会员
联系人:茅勇
电话:021-60513351 拨打
手机:18049719720 拨打
邮箱:13851015143@163.com

产品详情:

英文名称:
DNA Damage Antibody Sampler Kit
供应商:
科敏商城
数量:
大量
规格:
1 Kit (7 x 20 ul)
保质期:
一年
保存条件:
低温
货号:
DNA Damage Antibody Sampler Kit
英文名:
DNA Damage Antibody Sampler Kit

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品牌:CST,产品名称:DNA Damage Antibody Sampler Kit #9947
英文名称:DNA Damage Antibody Sampler Kit
Product name:DNA Damage Antibody Sampler Kit #9947
Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.
Background: Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). p53 is phosphorylated by ATM, ATR and DNA-PK at Ser15. This phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,5). Chk1 and Chk2, downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint control, embryonic development and tumor suppression (6). Chk1 is phosphorylated at Ser280 and Ser296 following DNA damage. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues, including Thr68, each followed by glutamine (SQ or TQ motif). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (7-9). The breast cancer susceptibility proteins BRCA1 and BRCA2 are frequently mutated in cases of hereditary breast and ovarian cancers and have roles in multiple processes related to DNA damage, repair, cell cycle progression, transcription, ubiquitination and apoptosis. Numerous DNA-damage induced phosphorylation sites on BRCA1 have been identified, including serine 1524, and kinases activated in a cell cycle-dependent manner, including Aurora A and CDK2, can also phosphorylate BRCA1. IR, DNA and radiometric-induced DNA damage also results in rapid phosphorylation of the histone H2A family member H2A.X at Ser139 by ATM (10,11). Within minutes following DNA damage, Ser139-phosphorylated H2A.X localizes to sites of DNA damage at subnuclear foci (12).

Specificity / Sensitivity:

All antibodies in the DNA Damage Antibody Sampler Kit recognize their targets proteins only when modified at the indicated site.

Protocols
  • 2197:

    Western Blotting

    ,

    Immunoprecipitation (Magnetic)

    ,

    Immunohistochemistry (Paraffin)

    ,

    Flow

  • 2348:

    Western Blotting

    ,

    Immunofluorescence

    ,

    Flow

  • 2853:

    Western Blotting

  • 5883:

    Western Blotting

  • 7074:

    Western Blotting

  • 7076:

    Western Blotting

  • 9009:

    Western Blotting

  • 9286:

    Western Blotting

    ,

    Immunofluorescence

    ,

    Flow

  • 9718:

    Western Blotting

    ,

    Immunohistochemistry (Paraffin)

    ,

    Immunofluorescence

    ,

    Flow

Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
Phospho-ATR (Ser428) Antibody 285320 µl
  • WB
H M R Mk300Rabbit 
Phospho-BRCA1 (Ser1524) Antibody 900920 µl
  • WB
H220Rabbit 
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb 219720 µl
  • WB
  • IP
  • IHC
  • F
H62Rabbit IgG
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb 234820 µl
  • WB
  • IF
  • F
H M R Mk56Rabbit IgG
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb 971820 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk15Rabbit IgG
Phospho-p53 (Ser15) (16G8) Mouse mAb 928620 µl
  • WB
  • IF
  • F
H53Mouse IgG1
Phospho-ATM (Ser1981) (D6H9) Rabbit mAb 588320 µl
  • WB
H350Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076100 µl
  • WB
Horse 
Product Description:

This kit provides an economical means to analyze major signaling checkpoints in response to DNA damage. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.

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温馨提示:不可用于临床治疗。
DNA Damage Antibody Sampler Kit;试剂

公司简介


成立日期 (13年)
注册资本 50万人民币
员工人数
年营业额
经营模式
主营行业

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