Blasticidin

选择性抗生素Blasticidin | invivogenblasticidin是一种高效的选择性抗生素，作用于真核细胞和原核细胞。稻瘟菌素是一类肽核苷类抗生素链霉菌griseochromogenes，干扰在核糖体的肽键的形成，从而抑制蛋白质的合成分离。

抗稻瘟是赋予了稻瘟抗性基因从Bacillus cereus（BSR），其编码灭瘟素脱氨酶。

稻瘟菌素诱导细胞死亡和细胞快速进行稻瘟抗性基因可以在不到一周的时间了。

通常情况下，哺乳动物细胞对稻瘟μ1-10μg／ml浓度敏感，和细菌的25-100μ克/毫升

Product concentration: 10 mg/ml

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 95% (HPLC)
Endotoxin level: < 1 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in blasticidin-sensitive and blasticidin-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in blasticidin-resistant cells
Blasticidin hydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer. This product is available in four pack sizes:
- ant-bl-05: 5 x 1 ml (50 mg)
- ant-bl-1: 10 x 1 ml (100 mg)
- ant-bl-5: 50 x 1 ml (500 mg)
- ant-bl-5b: 1 x 50ml (500 mg)
Blasticidin hydrochloride is also supplied as a powder:
-  ant-bl-10p: 1 g (powder)
Blasticidin is shipped at room temperature. Upon receipt it should be stored at 4°C or -20°C.

推荐使用浓度
大肠杆菌
大肠杆菌对Blasticidin不敏感，但有Blasticidin抗性的转化子可以在低盐LB琼脂糖培养基，pH8,100ug/ml Blasticidin的条件下筛选。高pH可增强Blasticidin的活性，invivogen公司的Fast-Media® Blas培养基可助您得到筛选效果。
哺乳动物细胞

质量控制：
纯度>95%(HPLC)
经细菌和哺乳动物细胞系鉴定活性