Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CaMKII delta antibody detects CaMKII delta protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
CaMKII delta antibody (GTX111401) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CaMKII delta antibody detects CaMKII delta protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CaMKII delta antibody (GTX111401) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of CaMKII delta protein from HepG2 whole cell extracts using 5 μg of CaMKII delta antibody (GTX111401).
Western blot analysis was performed using CaMKII delta antibody (GTX111401).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
CaMK2D antibody detects CaMK2D protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
C. 30 µg HCT116 whole cell lysate/extract
10% SDS-PAGE
CaMK2D antibody (GTX111401) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CaMKII delta antibody detects CaMKII delta protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CaMKII delta protein stained by CaMKII delta antibody (GTX111401) diluted at 1:500.
Blue: Hoechst 33342 staining.