基本信息 产品详情 公司简介 推荐产品
Western抗体洗脱液

Western抗体洗脱液

询价250ml起订
湖北 2022-01-11

康迪斯化工(湖北)有限公司

VIP3年

产品详情:

中文名称:
Western抗体洗脱液
CAS号:
保存条件:
常温储存
纯度规格:
99%
产品类别:
试剂
含量:
99%
包装:
250ml/瓶
外观:
液体

Stripping is the term used to describe the removal of primary and secondary antibodies from a western blot membrane. Stripping is useful when one wants to investigate more than one protein on the same blot, for instance a protein of interest and a loading control. When probing for multiple targets, stripping and re-probing a single membrane instead of running and blotting multiple gels has the advantage of saving samples, materials, and time.

It is not advisable to make quantitative comparisons of targets probed before and after stripping since the procedure removes some sample protein from the membrane. For the same reason, a stripped membrane should not be probed to demonstrate the absence of a protein.

A PVDF membrane is highly recommended to minimize loss of sample protein. Note also that colorimetric/chromogenic detection reagents will leave a permanent visible stain on the membrane that can interfere with subsequent detection of targets of similar molecular weights. Chemiluminescent reagents such as ECL are recommended as they will not leave a stain and are more sensitive than colorimetric reagents.

Procedure

1.Warm the buffer to 50°C
2.Add the buffer to a small plastic box which has a tight lid; use a volume that will cover the membrane
3.Add the membrane. Incubate at 50°C for up to 45 min with some agitation
4.Dispose of the solution as required for ß-mercaptoethanol based buffers
5.Rinse the membrane under running water tap for 1–2 min
6.Traces of ß-mercaptoethanol will damage the antibodies. Wash extensively for 5 min in TBST
7.Ready for blocking

 

 

注意事项:
1. 膜再生液实质是在不影响膜上结合的抗原的条件下将抗体洗脱下来,实际操作中,膜类型、抗体类型、浓度及抗原特性都影响洗脱难易度。
2. 适用于 ECL 和类似的化学发光试剂进行的 Western 检测。不适用于非化学发光试剂进行的 Western检测,例如 DAB,NBT/BCIP。
3. 用 ECL 显色后,再生液洗脱几分钟后,可再次加入 ECL 显色液显影,如胶片上显示条带,表明抗体未除净,须进一步在再生液中浸泡,至胶片上无显色。
4. 使用脱脂奶粉封闭的膜要比使用 BSA 封闭的膜更容易再生。
5. 尽量避免让膜干燥。

 

在遇到一抗二抗去除效果欠佳时,可以考虑适当延长一抗二抗去除液的孵育时间,同时确保孵育时的温度不低于20℃。温度过低时一抗二抗的去除效果会下降。同时也可以考虑尝试其它的一抗二抗去除液,以摸索出最佳的适合您实验条件的Western一抗二抗去除液。


 

 

 

我们所提供的产品仅供科研使用,不得用于临床诊断、治疗、食品或者化妆品等用途!在您向我们订购产品之时,表明您已经知晓我们的产品仅做科研用途,并遵守法律法规!

Western抗体洗脱液

公司简介

康迪斯化工(湖北)有限公司是一家立足于生命科学领域有自主知识产权的高新技术企业,主要从事生物试剂的研发与生产,并以持续探索和开发生命科学技术为动力,致力于为科研工作者提供实用、高效的科研试剂,为生命科学的研究与发展提供有意义的帮助。

Western抗体洗脱液相关厂家报价 更多

内容声明:
以上所展示的信息由商家自行提供,内容的真实性、准确性和合法性由发布商家负责。 商家发布价格指该商品的参考价格,并非原价,该价格可能随着市场变化,或是由于您购买数量不同或所选规格不同而发生变化。最终成交价格,请咨询商家,以实际成交价格为准。请意识到互联网交易中的风险是客观存在的
拨打电话 委托采购