饱和硫酸铵溶液

产品简介：

硫酸铵(Ammonium sulfate)分子量为132.14，化学式为(NH4)2·SO4，CAS号为7783-20-2，饱和硫酸铵溶液多用于硫酸铵盐析法沉淀蛋白质。硫酸铵盐析法是分离蛋白质常用的方法之一，其原理是当高浓度的盐加入到蛋白溶液中，铵离子和硫酸根离子同蛋白质竞争与水结合，使蛋白质的溶解性降低而从水溶液中沉淀析出。饱和硫酸铵溶液(SAS)pH为7.0～7.2，操作简单，不易引起蛋白质变性失活，其缺点是所得抗体纯度不高，易受其他蛋白质污染。

试剂保存：室温保存，一年有效。

操作步骤(仅供参考)：按实验具体要求操作。

注意事项：为了您的安全和健康，请穿实验服并戴一次性手套操作。

Ammonium sulphate precipitation is the most commonly used salt ppt because it is highly soluble in water, it stabilizes most proteins in solution (although it may denature some MAbs), and helps reduce the lipid content of the sample.

Ammonium sulphate ppt removes up to 50% of contaminating protein, and therefore reduces the load for subsequent chromatography. Most precipitated proteins retain their activity and native conformation, and can be redissolved easily. Ideal for crude and dilute samples.

TO MAKE A SOLUTION 20% IN AMMONIUM SULFATE (FROM 100% STOCK) ADD 0.25 VOLUMES OF 100% AMMONIUM SULFATE.

Here's an example: Say you have 3 mls of a solution and want to make it 20% ammonium sulfate. The final volume will be 3 mls + x mls where x is the amount of ammonium sulfate you need to add.

Using:
(Final V) x (final C) = (V of concentrate added) x (concentrate C).

Then:
(3 + x) x (20%) = (x) x (100%)
60 + 20x = 100x
60 = 80x
x = 3/4 or 0.75 ml.
so, to bring a 3 ml volume to 20% ammonium sulfate you will need to add 0.75 mls of 100% concentrate.

TO MAKE A SOLUTION 40% IN AMMONIUM SULFATE (FROM 100% STOCK) ADD 0.67 VOLUMES OF 100%% AMMONIUM SULFATE.
(3 + x) x (40%) = (x) x (100%)
120 + 40x = 100x
120 = 60x
x = 2 mls (or 0.67 volumes).

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