杜氏磷酸缓冲液

名称 杜氏磷酸缓冲液 (1×DPBS,无钙镁) Dulbecco's Phosphate Buffered Saline,without Calcium-Magnesium

规格 500mL

存储 4℃保存6个月有效。室温下可短期存储。

产品简介

杜氏磷酸缓冲液 (1×DPBS,无钙镁) Dulbecco's Phosphate Buffered Saline,without Calcium-Magnesium 是最常用的磷酸盐缓冲溶液之一，又称Dulbecco's磷酸缓冲盐溶液、Dulbecco's PBS、D-PBSA、CMF-DPBS、D-PBS、DPBS, 主要由氯化钠、氯化钾、磷酸氢二钠、磷酸二氢钾组成，不含Ca2+、Mg2+，pH值为7.4，该试剂经过高压灭菌，常用于细胞培养过程中细胞的洗涤或其他常规用途。该试剂为1×工作液，直接使用。

注意事项

1、在进行细胞培养过程中细胞的洗涤时，应注意无菌操作，避免被微生物污染。

2、该试剂经高压灭菌处理。

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Dulbecco′s Phosphate Buffered Saline has been used for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate. It has also been used to wash the wells of microtiter plate

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