SYBR Green II RNA

l Analyze small aliquots from RNA preparations before Northern blotting, start-site mapping or cDNA preparation

l Visualize the migration behavior of 5S rRNA species after high-resolution denaturing gradient electrophoresis (DGGE)

l Stain DNA in single-strand conformation polymorphism (SSCP)

l Stain DNA before amplification by PCR

Materials Contents

SYBR Green II stain is provided as a 10,000X concentrate in DMSO in the following sizes:

l 50 μL (SY1040-50)

l 500 μL (SY1040-500)

Storage

Upon receipt, store the dye frozen at -20°C, protected from light in a desiccator. When stored properly, the SYBR Green II stain stock solution in DMSO is stable for six months to one year.

注：SYBR Green II主要用于RNA和单链DNA电泳。是EB的替代品，无致癌性，超敏感性。用作常规电泳时，须将商品化的核酸MARKER作一定倍数的稀释（1/5-1/10），这样才能得到比较理想的结果。