产品编号 |
产品名称 |
产品包装 |
产品价格 |
AF0060 |
Bcl-2 Rabbit Polyclonal Antibody |
50μl |
416.00元 |
来源
|
用途
|
交叉反应性
|
分子量
|
Rabbit
|
WB, FC, IHC, ICC, IF
|
H
|
26KDa
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
1:500-1:1000
|
-
|
1:200
|
1:200
|
1:200
|
1:50-1:100
|
-
|
-
|
抗体详细信息如下:
About this Antibody
|
Name
|
Bcl-2 Rabbit Polyclonal Antibody
|
Category
|
Polyclonal antibody(pAb); Primary antibody
|
Isotype
|
IgG
|
Purification
|
Peptide affinity purified
|
About the Immunogen
|
Immunogen
|
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human Bcl-2.
|
Gene ID
|
596
|
SwissProt
|
P10415
|
Synonyms
|
Bcl-2; PPP1R50
|
Category
|
Apoptosis
|
Background
|
Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments. Antibodies to Bcl-2 can be used with immunohistochemistry to identify cells containing the antigen. In healthy tissue, these antibodies will react with B-cells in the mantle zone, as well as some T-cells. However, there is a considerable increase in positive cells in follicular lymphoma, as well as many other forms of cancer. In some cases, the presence or absence of Bcl-2 staining in biopsies may be significant for the patient's prognosis or likelihood of relapse.
|
包装清单:
产品编号
|
产品名称
|
包装
|
AF0060
|
Bcl-2 Rabbit Polyclonal Antibody
|
50μl
|
AZ050
|
Western一抗稀释液
|
50ml
|
—
|
说明书
|
1份
|
保存条件:
Bcl-2 Rabbit Polyclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。