产品编号 |
产品名称 |
产品包装 |
产品价格 |
AF0216 |
LDHA Rabbit Polyclonal Antibody |
50μl |
416.00元 |
来源 |
用途 |
交叉反应性 |
分子量 |
Rabbit |
WB, IHC, FC |
H, M, R |
37KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
1:500-1:1000 |
- |
- |
1:200 |
- |
1:100-1:200 |
- |
- |
抗体详细信息如下:
About this Antibody |
Name |
LDHA Rabbit Polyclonal Antibody |
Category |
Polyclonal antibody(pAb); Primary antibody |
Isotype |
IgG |
Purification |
Peptide affinity purified |
About the Immunogen |
Immunogen |
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of LDHA. |
Gene ID |
3939 |
SwissProt |
P00338 |
Synonyms |
LDHA; LDH-M; LDH1; PIG19 |
Category |
Metabolism |
Background |
Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor. |
包装清单:
产品编号 |
产品名称 |
包装 |
AF0216 |
LDHA Rabbit Polyclonal Antibody |
50μl |
AZ050 |
Western一抗稀释液 |
50ml |
— |
说明书 |
1份 |
保存条件:
LDHA Rabbit Polyclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。