产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5450-100μg |
Recombinant Human aFGF, 2-155a.a. |
100μg |
1375.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
2246
|
P05230
|
E. coli
|
155aa
|
17.5kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human aFGF, 2-155a.a. (Recombinant Human Acidic Fibroblast Growth Factor, 2-155a.a.; rHuaFGF, 2-155a.a.);重组人酸性成纤维细胞生长因子,2-155a.a.
|
Synonyms
|
Fibroblast growth factor 1; FGF-1; Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1; FGFA
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×106IU/mg in the presence of 10μg/ml of heparin.
|
Physical Appearance
|
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 2mM EDTA, 0.5mM DTT, 5% Trehalose.
|
Endotoxin
|
Less than 0.1EU/μg of rHuaFGF, 2-155a.a. as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Human aFGF, encoded by the FGF1 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Human aFGF shares 54% amino acid sequence identity with bFGF. In mammalian FGF receptor family has 4 members, FGFR1, FGFR2, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types "b", "c". aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. aFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. aFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion.
|
Amino Acid Sequence
|
MAEGEITTFT ALTEKFNLPP GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESVGEVY IKSTETGQYL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5450-10μg
|
Recombinant Human aFGF, 2-155a.a.
|
10μg
|
P5450-50μg
|
Recombinant Human aFGF, 2-155a.a.
|
50μg
|
P5450-100μg
|
Recombinant Human aFGF, 2-155a.a.
|
100μg
|
P5450-1mg
|
Recombinant Human aFGF, 2-155a.a.
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂,此时冻干后本产品看起来仍然像是液体,属于正常现象。在冻干工艺上,海藻糖作为常用的冻干保护剂,可显著阻止蛋白质二级结构的改变,防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。