产品编号 |
产品名称 |
产品包装 |
产品价格 |
P7280-1mg |
Recombinant Human ApoA-I |
1mg |
5665.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
335
|
P02647
|
E. coli
|
243aa
|
28.1kDa
|
-
|
About this protein
|
Name
|
Recombinant Human ApoA-I (Recombinant Human Apolipoprotein A-I; rHuApoA-I);重组人载脂蛋白A-I
|
Synonyms
|
Alp-1; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; APOA1; Apo-AI; apo-AI; ApoA-I; apoA-I; Apolipoprotein A1; apolipoprotein A-I; MGC117399
|
Purity
|
>98% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Data Not Available.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.
|
Endotoxin
|
Less than 0.1EU/μg of rHuApoA-I as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Others
|
Background
|
Apolipoprotein A-I encoded by the APOA1 gene is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Apolipoprotein A-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Apolipoprotein A-I is also isolated as a prostacyclin (PGI2) stabilizing factor and thus may have an anticlotting effect. Apolipoprotein A-I has been shown to interact with ABCA1, GPLD1 and PLTP. Apolipoprotein A-I binds to lipopolysaccharide or endotoxin and has a major role in the anti-endotoxin function of HDL. Mature human ApoA-1 shares 65% and 62%a.a. sequence identity with murine and rat ApoA-1, respectively. It has an approximate molecular weight of 28.1kDa.
|
Amino Acid Sequence
|
DEPPQSPWDR VKDLATVYVD VLKDSGRDYV SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SALEEYTKKL NTQ
|
包装清单:
产品编号
|
产品名称
|
包装
|
P7280-20μg
|
Recombinant Human ApoA-I
|
20μg
|
P7280-100μg
|
Recombinant Human ApoA-I
|
100μg
|
P7280-1mg
|
Recombinant Human ApoA-I
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。