| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P5336-1mg |
Recombinant Human BAFF/TNFSF13B |
1mg |
26773.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
10673
|
Q9Y275
|
E. coli
|
153aa
|
17.2kDa
|
－
|
|
About this protein
|
|
Name
|
Recombinant Human BAFF/TNFSF13B (Recombinant Human B Cell Activating Factor; rHuBAFF)；重组人B细胞激活因子/TNFSF13B
|
|
Synonyms
|
BLyS; CD257; TALL1; THANK; TNFSF13B; ZTNF4; ApoL related ligand TALL-1; B lymphocyte stimulator; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257; CD257 antigen; Dendritic cell-derived TNF-like molecule; DTL; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
|
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2μg/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
|
|
Endotoxin
|
Less than 1EU/μg of rHuBAFF as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
B-cell activating factor (BAFF), also known as Blys, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56%a.a. sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.
|
|
Amino Acid Sequence
|
MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P5336-5μg
|
Recombinant Human BAFF/TNFSF13B
|
5μg
|
|
P5336-20μg
|
Recombinant Human BAFF/TNFSF13B
|
20μg
|
|
P5336-100μg
|
Recombinant Human BAFF/TNFSF13B
|
100μg
|
|
P5336-1mg
|
Recombinant Human BAFF/TNFSF13B
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
