Recombinant Human EGF

Recombinant Human EGF
62229-50-9

上海更新日期：2025-03-07

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产品详情:

中文名称：: Recombinant Human EGF

英文名称：: Recombinant Human EGF

CAS号：: 62229-50-9

产品类别：: 重组蛋白细胞因子

产品编号	产品名称	产品包装	产品价格
P5552-1mg	Recombinant Human EGF	1mg	482.00元

Species	Gene ID	Accession	Source	Length	MW	Tag
Human	1950	P01133	E. coli	53aa	6.2kDa	－

About this Protein
Name	Recombinant Human EGF (Recombinant Human Epidermal Growth Factor; rHuEGF)；重组人表皮生长因子
Synonyms	HOMG4; URG; Urogastrone; beta-urogastrone; epidermal growth factor; epidermal growth factor (beta-urogastrone); HOMG4; pro-epidermal growth factor; URG
Purity	>98% by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×10⁷IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin	Less than 1EU/μg of rHuEGF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category	Cytokine
Background	Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature human EGF shares 70%a.a. sequence identity with mature mouse and rat EGF.
Amino Acid Sequence	NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

包装清单:

产品编号	产品名称	包装
P5552-100μg	Recombinant Human EGF	100μg
P5552-500μg	Recombinant Human EGF	500μg
P5552-1mg	Recombinant Human EGF	1mg
-	说明书	1份

保存条件:
       -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
       由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
       本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
       为了您的安全和健康，请穿实验服并戴一次性手套操作。

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公司简介

碧云天由美国哈佛大学的留学人员创办于2001年，为上海市高新技术企业，公司核心团队由来自美国哈佛大学、NIH、UCLA、香港大学、南京大学、中国科技大学、中国科学院等著名大学和科研机构的高水平科研人员及默克、诺华等顶尖医药企业的管理人员组成。十多年来，碧云天已经成为世界一流的生物、医学研究用试剂、试剂盒和消耗品的研发和生产企业，同时提供生命科学研究的技术服务和一站式实验仪器设备采购平台。目前已有近50000篇注明使用碧云天产品的研究论文发表在包括Nature、Cell等国际高水平学术期刊，日均逾33.37篇！碧云天将继续致力于科研用技术和产品的研发，用我们最顶尖的技术、最成熟的产品、最热情的服务，服务科研人员，造福生命健康

成立日期	(18年)
注册资本	1000.000000万人民币
员工人数	500人以上
年营业额	￥ 500万-1000万
经营模式	试剂
主营行业	生化试剂,核苷，核苷酸，寡核苷酸,抗体,蛋白组学,生物活性小分子