产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5300-2000IU |
Recombinant Human EPOα |
2000IU |
1152.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
2056
|
P01588
|
CHO
|
166aa
|
36~40kDa
|
-
|
About this protein
|
Name
|
Recombinant Human EPOα (Recombinant Human EPOα; rHuEPOα, Liquid);重组人促红细胞生成素α
|
Synonyms
|
EPO; EP; epoetin; Epoetin; erythropoietin; MGC138142; MVCD2
|
Purity
|
>98% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and was found to be no less than 1.5×105IU/mg.
|
Physical Appearance
|
Clear colorless liquid.
|
Formulation
|
Sterile filtered liquid in sodium citrate buffer (1 liter of ddH2O containing 5.9g of sodium citrate, 5.8g of sodium chloride and 0.06g of citric acid).
|
Endotoxin
|
Less than 0.01EU/μg of rHuEPOα as determined by LAL method.
|
Reconstitution
|
N/A
|
Category
|
Cytokine
|
Background
|
Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients.
The cDNAs for EPO have been cloned from human, murine, canine, etc. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level. Human EPO cDNA encodes a 193 amino acid residue precursor protein that is processed to yield a 165 amino acid residue mature protein. EPO contains one O-linked and three N-linked glycosylation sites. Glycosylation of EPO is required for EPO biological activities in vivo. EPO exhibits structural as well as amino sequence identity to the amino terminal 153 amino acid region of thrombopoietin.
|
Amino Acid Sequence
|
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5300-500IU
|
Recombinant Human EPOα
|
500IU
|
P5300-2000IU
|
Recombinant Human EPOα
|
2000IU
|
P5300-15000IU
|
Recombinant Human EPOα
|
15000IU
|
P5300-150000IU
|
Recombinant Human EPOα
|
150000IU
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。