产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5498-5μg |
Recombinant Human FGF-23 |
5μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
8074
|
Q9GZV9
|
E. coli
|
227aa
|
25.3kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human FGF-23 (Recombinant Human Fibroblast Growth Factor-23; rHuFGF-23);重组人成纤维细胞生长因子23
|
Synonyms
|
ADHR; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; Phosphatonin; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/ml, corresponding to a specific activity of >2.0×103IU/mg in the presence of 0.3μg/ml of rMuKlotho and 10μg/ml of heparin.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
Endotoxin
|
Less than 1EU/μg of rHuFGF-23 as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Human FGF-23 belongs to the FGF-19 subfamily which has three members FGF-19, 21, 23. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. They are classically considered to be paracrine factors and are known for their roles in tissue patterning and organogenesis during embryogenesis. By contrast, the FGF-19 subfamily has recently been shown to function in an endocrine manner. Members of this subfamily have poor ability of binding to heparin binding site which is a crucial factor in ligand-receptor complex formation. β-Klotho has been identified as co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligand-receptor affinity. FGF-23 is most highly expressed in bone, from which it circulates through the blood to regulate vitamin D and phosphate metabolism in kidney.
|
Amino Acid Sequence
|
YPNASPLLGS SWGGLIHLYT ATARNSYHLQ IHKNGHVDGA PHQTIYSALM IRSEDAGFVV ITGVMSRRYL CMDFRGNIFG SHYFDPENCR FQHQTLENGY DVYHSPQYHF LVSLGRAKRA FLPGMNPPPY SQFLSRRNEI PLIHFNTPIP RRHTRSAEDD SERDPLNVLK PRARMTPAPA SCSQELPSAE DNSPMASDPL GVVRGGRVNT HAGGTGPEGC RPFAKFI
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5498-5μg
|
Recombinant Human FGF-23
|
5μg
|
P5498-20μg
|
Recombinant Human FGF-23
|
20μg
|
P5498-100μg
|
Recombinant Human FGF-23
|
100μg
|
P5498-1mg
|
Recombinant Human FGF-23
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。