产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5637-20μg |
Recombinant Human IFN-α1a |
20μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
3439
|
P01562
|
E. coli
|
167aa
|
19.5kDa
|
-
|
About this Protein
|
Name
|
Recombinant Human IFN-α1a (Recombinant Human Interferon-α1a; rHuIFN-α1a);重组人干扰素α1a
|
Synonyms
|
IFL; IFN; IFNA; IFNA13; IFN-ALPHA; IFN-alphaD
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0×108IU/mg.
|
Physical Appearance
|
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4, containing 3% Mannitol, 5% Trehalose, 0.05% Tween-80.
|
Endotoxin
|
Less than 0.1EU/μg of rHuIFN-α1a as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Interferon alpha-1, also named interferon alpha-D, is belonging to the type I interferon family and is encoded by the IFNA1gene in humans. Interferon alpha-1 is expressed in peripheral blood leukocytes and lymphoblastoid cells. IFNα-1 contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. It plays an important role in inducing non-specific resistance against a broad range of viral infections, and also affects cell proliferation and modulates immune responses.
IFNα-1a and IFNα-1b are two forms of interferon alpha-1 except for the substitution of alanine for valine at position 137. Interferon-alpha represents a group of related but distinct proteins that share over 95% amino acid sequence homology.
|
Amino Acid Sequence
|
MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNADSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5637-20μg
|
Recombinant Human IFN-α1a
|
20μg
|
P5637-100μg
|
Recombinant Human IFN-α1a
|
100μg
|
P5637-1mg
|
Recombinant Human IFN-α1a
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂,此时冻干后本产品看起来仍然像是液体,属于正常现象。在冻干工艺上,海藻糖作为常用的冻干保护剂,可显著阻止蛋白质二级结构的改变,防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。