| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P5520-1mg |
Recombinant Human IGF-BP5 |
1mg |
15444.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
3488
|
P24593
|
E. coli
|
252aa
|
28.6kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Human IGF-BP5 (Recombinant Human Insulin-like Growth Factor-Binding Protein 5; rHuIGF-BP5)；重组人胰岛素样生长因子结合蛋白5
|
|
Synonyms
|
IBP5IBP-5; IGF-binding protein 5; IGFBP-5; insulin-like growth factor binding protein 5; insulin-like growth factor-binding protein 5
|
|
Purity
|
>96% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4μg/ml, corresponding to a specific activity of >2500IU/mg in the presence of 15ng/ml of rHuIGF-II.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 10mM Sodium Citrate, pH3.0.
|
|
Endotoxin
|
Less than 0.1EU/μg of rHuIGF-BP5 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxyterminal thirds of the molecule. IGF-BP5 is produced by vascular smooth muscle cells. It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts. IGFBP-5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP-5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development. Recombinant human IGFBP5 is a 28.6kDa protein consisting of 252 amino acid residues. Human and murine IGF-BP5 share 97%a.a. sequence identity.
|
|
Amino Acid Sequence
|
LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P5520-5μg
|
Recombinant Human IGF-BP5
|
5μg
|
|
P5520-25μg
|
Recombinant Human IGF-BP5
|
25μg
|
|
P5520-100μg
|
Recombinant Human IGF-BP5
|
100μg
|
|
P5520-1mg
|
Recombinant Human IGF-BP5
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
