产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5259-100μg |
Recombinant Human IL-36β, 153a.a. |
100μg |
4759.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
27177
|
Q9NZH7
|
E. coli
|
153aa
|
17.2kDa
|
-
|
About this protein
|
Name
|
Recombinant Human IL-36β, 153a.a. (Recombinant Human Interleukin-36β, 153a.a.; rHuIL-36β, 153a.a.);重组人白细胞介素36β,153a.a.
|
Synonyms
|
IL36B; IL1F8; IL1H2
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10ng/ml, corresponding to a specific activity of >1.0×105IU/mg.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4.
|
Endotoxin
|
Less than 1EU/μg of rHuIL-36β, 153a.a. as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36 beta is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin and it can stimulate production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. It has two isoforms. IL-36β isoform 2 contains one potential N-linked glycosylation site in its C-terminus, while IL-36β isoform 1 lacks potential N-linked glycosylation sites and four of the conserved β-strands. Human IL-36β isoform 2 shares 62%, 67%, 63% and 59%a.a. identity with the most similar isoform of mouse, canine, bovine and equine IL-36β, respectively.
|
Amino Acid Sequence
|
REAAPKSYAI RDSRQMVWVL SGNSLIAAPL SRSIKPVTLH LIACRDTEFS DKEKGNMVYL GIKGKDLCLF CAEIQGKPTL QLKEKNIMDL YVEKKAQKPF LFFHNKEGST SVFQSVSYPG WFIATSTTSG QPIFLTKERG ITNNTNFYLD SVE
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5259-2μg
|
Recombinant Human IL-36β, 153a.a.
|
2μg
|
P5259-10μg
|
Recombinant Human IL-36β, 153a.a.
|
10μg
|
P5259-100μg
|
Recombinant Human IL-36β, 153a.a.
|
100μg
|
P5259-1mg
|
Recombinant Human IL-36β, 153a.a.
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。