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网站主页 RECOMBINANT HUMAN LIF Recombinant Human LIF
  • Recombinant Human LIF

Recombinant Human LIF

Recombinant Human LIF
上海 更新日期：2024-12-25

碧云天生物技术有限公司

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产品详情:

中文名称：
Recombinant Human LIF
英文名称：
Recombinant Human LIF
产品类别：
重组蛋白 细胞因子
产品编号 产品名称 产品包装 产品价格
P5354-1mg Recombinant Human LIF 1mg 14411.00元
Species Gene ID Accession Source Length MW Tag
Human 3976 P15018 E. coli 180aa 19.7kDa －
About this protein
Name Recombinant Human LIF (Recombinant Human Leukemia Inhibitory Factor; rHuLIF)；重组人白血病抑制因子
Synonyms CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); Melanoma-derived LPL inhibitor; MLPLI
Purity >98% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.
Endotoxin Less than 1EU/μg of rHuLIF as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.2mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Cytokine
Background Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature human LIF (180a.a.) shares 78%, 82%, 91%, 88% and 87%a.a. sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively.
Amino Acid Sequence SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

包装清单:

产品编号 产品名称 包装
P5354-5μg Recombinant Human LIF 5μg
P5354-25μg Recombinant Human LIF 25μg
P5354-100μg Recombinant Human LIF 100μg
P5354-1mg Recombinant Human LIF 1mg
- 说明书 1份

保存条件:
       -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
       由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
       本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
       为了您的安全和健康，请穿实验服并戴一次性手套操作。

Recombinant Human LIF

公司简介

碧云天由美国哈佛大学的留学人员创办于2001年，为上海市高新技术企业，公司核心团队由来自美国哈佛大学、NIH、UCLA、香港大学、南京大学、中国科技大学、中国科学院等著名大学和科研机构的高水平科研人员及默克、诺华等顶尖医药企业的管理人员组成。十多年来，碧云天已经成为世界一流的生物、医学研究用试剂、试剂盒和消耗品的研发和生产企业，同时提供生命科学研究的技术服务和一站式实验仪器设备采购平台。目前已有近50000篇注明使用碧云天产品的研究论文发表在包括Nature、Cell等国际高水平学术期刊，日均逾33.37篇！碧云天将继续致力于科研用技术和产品的研发，用我们最顶尖的技术、最成熟的产品、最热情的服务，服务科研人员，造福生命健康

成立日期 (18年)
注册资本 1000.000000万人民币
员工人数 500人以上
年营业额 ￥ 500万-1000万
经营模式 试剂
主营行业 生化试剂,核苷，核苷酸，寡核苷酸,抗体,蛋白组学,生物活性小分子

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