产品编号 |
产品名称 |
产品包装 |
产品价格 |
P7140-1mg |
Recombinant Human MIC-A |
1mg |
12354.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
100507436
|
Q29983
|
E. coli
|
284aa
|
32.8kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human MIC-A (Recombinant Human MHC Class I PolypeptideRelated Sequence A; rHuMIC-A);重组人MIC类多肽相关序列A
|
Synonyms
|
FLJ60820; MGC111087; PERB11.1
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile, 0.1% TFA.
|
Endotoxin
|
Less than 1EU/μg of rHuMIC-A as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration less than 0.2mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Others
|
Background
|
MIC-A (MHC class I chain-related gene A) is a single-pass type I member protein. It is expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Additionally, MIC-A can be induced by bacterial and viral infections. It shares 85% amino acid identity with MIC-B and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules. They do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ αβ T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases.
|
Amino Acid Sequence
|
EPHSLRYNLT VLSWDGSVQS GFLAEVHLDG QPFLRYDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTVP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLESGVVLRR TVPPMVNVTR SEASEGNITV TCRASSFYPR NIILTWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC RGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHW
|
包装清单:
产品编号
|
产品名称
|
包装
|
P7140-10μg
|
Recombinant Human MIC-A
|
10μg
|
P7140-50μg
|
Recombinant Human MIC-A
|
50μg
|
P7140-100μg
|
Recombinant Human MIC-A
|
100μg
|
P7140-1mg
|
Recombinant Human MIC-A
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。