产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5358-1mg |
Recombinant Human OSM |
1mg |
27053.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
5008
|
P13725
|
E. coli
|
227aa
|
25.8kDa
|
-
|
About this protein
|
Name
|
Recombinant Human OSM (Recombinant Human Oncostatin-M; rHuOSM);重组人肿瘤抑制素M
|
Synonyms
|
MGC20461; oncostatin M; oncostatin-M
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
Endotoxin
|
Less than 1EU/μg of rHuOSM as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant human OSM has approximately 40% and 39% amino acid sequence identity with rat and murine OSM.
|
Amino Acid Sequence
|
AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRRT RPSRKGKRLM TRGQLPR
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5358-2μg
|
Recombinant Human OSM
|
2μg
|
P5358-10μg
|
Recombinant Human OSM
|
10μg
|
P5358-100μg
|
Recombinant Human OSM
|
100μg
|
P5358-1mg
|
Recombinant Human OSM
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。