产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5736-5μg |
Recombinant Human PTN |
5μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
5764
|
P21246
|
E. coli
|
136aa
|
15.3kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human PTN (Recombinant Human Pleiotrophin; rHuPTN);重组人多效生长因子
|
Synonyms
|
HARP; HBNF; HNGF-8; OSF-1; PTN; HARP; HBBM; HB-GAM; HBGF8; HBNF; HBNF1; HBNF-1; Heparin-binding brain mitogen; NEGF1HBGF-8; neurite growth-promoting factor 1; neurite growth-promoting factor1); OSF-1; Osteoblast-specific factor 1; pleiotrophin
|
Purity
|
>96% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been pre-coated with 100 μl/well of a solution of 5-10μg/ml rHuPTN.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
|
Endotoxin
|
Less than 0.1EU/μg of rHuPTN as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Pleiotrophin, also named heparin-binding brain mitogen (HBBM), is belonging to the heparin-binding proteins family and is encoded by the PTN gene in humans. Pleiotrophin is expressed in an activity-dependent manner in the hippocampus. Pleiotrophin expression is low in other areas of the adult brain, but it can be induced by ischemic insults. The cell surface-expressed nucleolin is a low affinity receptor for PTN binding to cells and it is also implicated in PTN entry into cells by an active process. Human pleiotrophin shares above 98% amino acid sequence identity with bovine, rat and murine.
|
Amino Acid Sequence
|
GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5736-5μg
|
Recombinant Human PTN
|
5μg
|
P5736-20μg
|
Recombinant Human PTN
|
20μg
|
P5736-100μg
|
Recombinant Human PTN
|
100μg
|
P5736-1mg
|
Recombinant Human PTN
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。