产品编号 |
产品名称 |
产品包装 |
产品价格 |
P7041-2μg |
Recombinant Human TIMP-2 |
2μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
7077
|
P16035
|
E. coli
|
194aa
|
21.8kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human TIMP-2 (Recombinant Human Tissue Inhibitor of Metalloproteinases 2; rHuTIMP-2);重组人组织金属蛋白酶抑制剂2
|
Synonyms
|
CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP-2; Tissue inhibitor of metalloproteinases 2
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit humanmMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-ARNH2.
|
Physical Appearance
|
Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 3% trehalose.
|
Endotoxin
|
Less than 1EU/μg of rHuTIMP-2 as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Enzymes
|
Background
|
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known asmMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin-like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation ofmMP-2 bymMP-14.
|
Amino Acid Sequence
|
CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP
|
包装清单:
产品编号
|
产品名称
|
包装
|
P7041-2μg
|
Recombinant Human TIMP-2
|
2μg
|
P7041-10μg
|
Recombinant Human TIMP-2
|
10μg
|
P7041-100μg
|
Recombinant Human TIMP-2
|
100μg
|
P7041-1mg
|
Recombinant Human TIMP-2
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂,此时冻干后本产品看起来仍然像是液体,属于正常现象。在冻干工艺上,海藻糖作为常用的冻干保护剂,可显著阻止蛋白质二级结构的改变,防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。