产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5331-100μg |
Recombinant Human TNF-β/TNFSF1 |
100μg |
4820.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
4049
|
P01374
|
E. coli
|
171aa
|
18.7kDa
|
-
|
About this protein
|
Name
|
Recombinant Human TNF-β/TNFSF1 (Recombinant Human Tumor Necrosis Factor-β/TNFSF1; rHuTNF-β/TNFSF1);重组人肿瘤坏死因子β/TNFS1
|
Synonyms
|
LTA; Lymphotoxin alpha; tnfb; TNF-beta; TNFSF1; TNFSF1B; LT; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); TNFBlymphotoxin-alpha; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1
|
Purity
|
>96% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5pg/ml, corresponding to a specific activity of >2.0×108IU/mg in the presence of actinomycin D.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20.
|
Endotoxin
|
Less than 0.1EU/μg of rHuTNF-β/TNFSF1 as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
TNF-beta, also known as lymphotoxin-alpha (LTalpha) and TNF-alpha are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical effects. Mature TNF-beta/LTalpha and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. Human and mouse TNF-beta/LTalpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. Soluble TNF-beta/LTalpha is a homotrimer in solution. Secreted TNFbeta/LTalpha also complexes with the membrane associated LTbeta/TNFSF3 to generate two types of heterotrimers, LTalpha1/beta2 and LTalpha2/beta1.
|
Amino Acid Sequence
|
LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5331-10μg
|
Recombinant Human TNF-β/TNFSF1
|
10μg
|
P5331-50μg
|
Recombinant Human TNF-β/TNFSF1
|
50μg
|
P5331-100μg
|
Recombinant Human TNF-β/TNFSF1
|
100μg
|
P5331-1mg
|
Recombinant Human TNF-β/TNFSF1
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。