产品编号 |
产品名称 |
产品包装 |
产品价格 |
P7262-20μg |
Recombinant Human Vimentin |
20μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
7431
|
P08670
|
E. coli
|
465aa
|
53.5kDa
|
-
|
About this protein
|
Name
|
Recombinant Human Vimentin (Recombinant Human Vimentin; rHuVimentin);重组人波形蛋白
|
Synonyms
|
VIM; FLJ36605; vimentin
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Data not available.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered solution in 30% Acetonitrile and 0.1% TFA.
|
Endotoxin
|
Less than 0.1EU/μg of rHuVimentin as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Others
|
Background
|
Vimentin is a type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells, so it is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Vimentin is a homopolymer assembled from elementary dimers which is mediated by the central alpha-helical coiled-coil rod region. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol and it is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Also, vimentin is found to control the transport of low-density lipoprotein, LDL, -derived cholesterol from alysosome to the site of esterification. Recombinant human vimentin contains 465 amino acid residues and it shares 97%a.a. sequence identity with murine and rat vimentin.
|
Amino Acid Sequence
|
STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE
|
包装清单:
产品编号
|
产品名称
|
包装
|
P7262-20μg
|
Recombinant Human Vimentin
|
20μg
|
P7262-100μg
|
Recombinant Human Vimentin
|
100μg
|
P7262-1mg
|
Recombinant Human Vimentin
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。