L-FABP; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5μM rMuFABP1 is achieved with approximately 5μM cis-paranaric acid.
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 2% trehalose.
Endotoxin
Less than 1EU/μg of rMuFABP1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids. These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes. Fatty acid-binding protein 1 (FABP1) encoded by the FABP1 gene, also known as liver-type fatty acid-binding protein (L-FABP), is a member of FABP family and it is a small, highly conserved, cytoplasmic proteins. In addition, FABP1 binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. Furthermore, it may be involved in intracellular lipid transport. Through amino acid sequence comparison, murine FABP1 shares 84% and 94%a.a. sequence identity with human and rat FABP1.