Recombinant Murine G-CSF

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

12985

P09920

E. coli

178aa

18.9kDa

－

About this Antibody

Name

Recombinant Murine G-CSF (Recombinant Murine Granulocyte Colony Stimulating Factor; rMuG-CSF)；Recombinant Mouse G-CSF ; 重组小鼠粒细胞集落刺激因子

Synonyms

CSF3; C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3OS; filgrastim; Filgrastim; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin; Pluripoietin

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 10mM Sodium Citrate, pH4.0, 150mM NaCl, 0.01% Tween-20.

Endotoxin

Less than 0.1EU/μg of rMuG-CSF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition, it may function in some adhesion or recognition events at the cell surface. The murine G-CSF cDNA encodes a 208 amino acid (a.a.) residue precursor protein containing a 30a.a. residue signal peptide that is proteolytically cleaved to generate the 178a.a. residue mature protein. Murine G-CSF is 73% identical at the amino acid level to human G-CSF and the two proteins show species cross-reactivity.

Amino Acid Sequence

VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

产品编号

产品名称

包装

P6002-2μg

Recombinant Murine G-CSF

2μg

P6002-10μg

Recombinant Murine G-CSF

10μg

P6002-100μg

Recombinant Murine G-CSF

100μg

P6002-1mg

Recombinant Murine G-CSF

1mg

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说明书

1份