Recombinant Murine IL-1RA

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

16181

P25085

E. coli

152aa

17.3kDa

－

About this Protein

Name

Recombinant Murine IL-1RA (Recombinant Murine Interleukin-1 Receptor Antagonist Protein; rMuIL-1RA)；Recombinant Mouse IL-1RA ; 重组小鼠白细胞介素1受体拮抗剂

Synonyms

IL-1F3; IL1RN; DIRA; ICIL-1RA; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50ng/ml, corresponding to a specific activity of >2.0×10⁴IU/mg in the presence of 50pg/ml rHuIL-1α.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuIL-1RA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The murine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26% amino acid homology to IL-1β and 19% homology to IL-1α. It also shares 90%a.a. sequence identity with rat IL-1RA.

Amino Acid Sequence

RPSGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNI KLEEKIDMVP IDLHSVFLGI HGGKLCLSCA KSGDDIKLQL EEVNITDLSK NKEEDKRFTF IRSEKGPTTS FESAACPGWF LCTTLEADRP VSLTNTPEEP LIVTKFYFQE DQ

产品编号

产品名称

包装

P5903-20μg

Recombinant Murine IL-1RA

20μg

P5903-100μg

Recombinant Murine IL-1RA

100μg

P5903-1mg

Recombinant Murine IL-1RA

1mg

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说明书

1份