Recombinant Murine IL-2

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

16183

P04351

E. coli

149aa

17.2kDa

－

About this Antibody

Name

Recombinant Murine IL-2 (Recombinant Murine Interleukin-2; rMuIL-2)；Recombinant Mouse IL-2 ; 重组小鼠白细胞介素2

Synonyms

aldesleukin; Aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2ng/ml, corresponding to a specific activity of >5.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuIL-2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4⁺ and CD8⁺ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55kDa IL-2Rα, 75kDa IL-2Rβ, 64kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Mature mouse IL-2 shares 56% and 73% amino acid sequence identity with human and rat IL-2 respectively. Mouse and human IL-2 exhibit cross-species activity. Mouse IL-2 has a specific N-terminal region that contains a poly glutamine (contain 12 glutamines) stretch.

Amino Acid Sequence

APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ

产品编号

产品名称

包装

P5907-5μg

Recombinant Murine IL-2

5μg

P5907-20μg

Recombinant Murine IL-2

20μg

P5907-100μg

Recombinant Murine IL-2

100μg

P5907-1mg

Recombinant Murine IL-2

1mg

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说明书

1份