Recombinant Murine M-CSF

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

12977

P07141

E. coli

230aa

52kDa

－

About this Antibody

Name

Recombinant Murine M-CSF (Recombinant Murine Macrophage Colony Stimulating Factor; rMuM-CSF)；Recombinant Mouse M-CSF ; 重组小鼠巨噬细胞集落刺激因子

Synonyms

CSF1; CSF-1; colony stimulating factor 1 (macrophage); CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; M-CSF; MCSFlanimostim; MGC31930

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in 20mM Tris, 500mM NaCl, pH7.4.

Endotoxin

Less than 1EU/μg of rMuM-CSF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150a.a. portion that is necessary and sufficient for interaction with the receptor. The first 229a.a. of mature mouse M-CSF shares 87%, 83%, 82% and 81% sequence identity with corresponding regions of rat, dog, cow and human M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Amino Acid Sequence

KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE

产品编号

产品名称

包装

P6015-2μg

Recombinant Murine M-CSF

2μg

P6015-10μg

Recombinant Murine M-CSF

10μg

P6015-100μg

Recombinant Murine M-CSF

100μg

P6015-1mg

Recombinant Murine M-CSF

1mg

-

说明书

1份