Recombinant Murine MANF

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

74840

Q3TMX5

E. coli

158aa

18.2kDa

－

About this Antibody

Name

Recombinant Murine MANF (Recombinant Murine Mesencephalic AstrocyteDerived Neurotrophic Factor; rMuMANF)；Recombinant Mouse MANF ; 重组小鼠中脑星形胶质细胞源性神经营养因子

Synonyms

ARMET; ARP; Arginine-Rich Protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10μg/ml, corresponding to a specific activity of >100IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 0.1EU/μg of rMuMANF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature murine MANF is 99%, 98% and 95%a.a. identical to mature rat, human and bovine MANF respectively.

Amino Acid Sequence

LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDNQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

产品编号

产品名称

包装

P6168-5μg

Recombinant Murine MANF

5μg

P6168-25μg

Recombinant Murine MANF

25μg

P6168-100μg

Recombinant Murine MANF

100μg

P6168-1mg

Recombinant Murine MANF

1mg

-

说明书

1份