Recombinant Murine Midkine

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

17242

P12025

E. coli

120aa

13.2kDa

－

About this Antibody

Name

Recombinant Murine Midkine (Recombinant Murine Midkine; rMuMidkine)；Recombinant Mouse Midkine ; 重组小鼠中期因子

Synonyms

ARAP; MDK; MEK; MK1; NEGF2; Amphiregulin-associated protein; Midgestation and kidney protein; midkine; midkine (neurite growth-promoting factor 2); MK1; MKARAP; NEGF2FLJ27379; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuMidkine as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. It is encoded by the MDK gene. The Midkine protein includes five intrachain disulfide bonds which hold two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.2kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87% identity between the human and murine proteins.

Amino Acid Sequence

VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD

产品编号

产品名称

包装

P6177-5μg

Recombinant Murine Midkine

5μg

P6177-20μg

Recombinant Murine Midkine

20μg

P6177-100μg

Recombinant Murine Midkine

100μg

P6177-1mg

Recombinant Murine Midkine

1mg

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说明书

1份