Recombinant Murine Neuropoietin

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

244218

P83714

E. coli

182aa

19.7kDa

－

About this Antibody

Name

Recombinant Murine Neuropoietin (Recombinant Murine Neuropoietin; rMuNeuropoietin)；Recombinant Mouse Neuropoietin ; 重组小鼠神经生成素

Synonyms

Cardiotrophin-2; CT-2; Neuropoietin; Np; Ctf2; Gm494

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 200ng/ml, corresponding to a specific activity of >5000IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH8.0, 500mM NaCl, with 0.5mM DTT.

Endotoxin

Less than 1EU/μg of rMuNeuropoietin as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Neuropoietin (NP; also known as cardiotrophin-2) is a 19.7kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 182 aa mature segment. The secreted molecule is characterized by the presence of four alpha-helices, typical of hematopoietic superfamily molecules. Mature murine neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively.

Amino Acid Sequence

APISPSEPIG QAYSLALYMQ KNTSALLQTY LQHQGSPFSD PGFSAPELQL STLPSAAVSF KTWHAMEDAE RLSRAQGAFL ALTQHLQLVG DDQSYLNPGS PILLAQLGAA RLRAQGLLGN MAAIMTALGL PIPPEEDTLG FVPFGASAFE RKCRGYIVTR EYGHWTDRAV RDLALLKAKY SA

产品编号

产品名称

包装

P6180-5μg

Recombinant Murine Neuropoietin

5μg

P6180-25μg

Recombinant Murine Neuropoietin

25μg

P6180-100μg

Recombinant Murine Neuropoietin

100μg

P6180-1mg

Recombinant Murine Neuropoietin

1mg

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说明书

1份