Recombinant Murine Noggin

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

18121

P97466

E. coli

206aa

46.4kDa

－

About this Antibody

Name

Recombinant Murine Noggin (Recombinant Murine Noggin; rMuNoggin)；Recombinant Mouse Noggin ; 重组小鼠生长因子

Synonyms

NOG; SYM1; SYNS1; noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×10⁵IU/mg in the presence of 5ng/ml BMP-4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μM filtered concentrated solution in 30% acetonitrile, 0.1% TFA.

Endotoxin

Less than 1EU/μg of rMuNoggin as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration less than 0.25mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Noggin encoded by the NOG gene, was first isolated from Xenopus, having the function of inducing secondary axis formation in frog embryos. It inhibits TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Additionally, it has pleiotropic effect, both in early development and later stages. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. In recent report, proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have relation with the mutant of evolutionarily conserved amino acid residues of Noggin. Mature mouse Noggin shares 99% and 83%a.a. sequence identity with human and Xenopus Noggin, respectively.

Amino Acid Sequence

MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

产品编号

产品名称

包装

P6191-5μg

Recombinant Murine Noggin

5μg

P6191-20μg

Recombinant Murine Noggin

20μg

P6191-100μg

Recombinant Murine Noggin

100μg

P6191-1mg

Recombinant Murine Noggin

1mg

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说明书

1份