Recombinant Murine PDGF-BB

Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

18591

P31240

E. coli

109aa

24.4kDa

－

About this Antibody

Name

Recombinant Murine PDGF-BB (Recombinant Murine Platelet-derived Growth Factor-BB; rMuPDGF-BB)；Recombinant Mouse PDGF-BB ; 重组小鼠血小板衍生生长因子-BB

Synonyms

Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Pdgfb; Sis

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 5.0.

Endotoxin

Less than 0.1EU/μg of rMuPDGF-BB as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). All PDGFs function as secreted, disulphide-linked homodimers, but only PDGF A and B can form functional heterodimers. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent in wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints in order to divide. Recombinant murine PDGF-AA contains 109 amino acids and has a molecular mass of 12.2kDa.

Amino Acid Sequence

SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TIVTPRPVT

产品编号

产品名称

包装

P6130-2μg

Recombinant Murine PDGF-BB

2μg

P6130-10μg

Recombinant Murine PDGF-BB

10μg

P6130-100μg

Recombinant Murine PDGF-BB

100μg

P6130-1mg

Recombinant Murine PDGF-BB

1mg

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说明书

1份