Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rMuProlactin as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Hormones
Background
Prolactin, also named PRL and lactotrope is a peptide hormone, encoded by the PRL gene. The structure of prolactin is similar to that of growth hormone and placental lactogen. There are mainly three different forms of prolactin in regard to size: little prolactin, big prolactin and big big prolactin. Little prolactin is the predominant form. Although often associated with human milk production, prolactin plays a wide range of other roles in both humans and other verterbrates. Recombinant murine prolactin is a 22.4kDa globular protein containing 197 amino acid residues and shares 77%~90%a.a. sequence identity with human and rat prolactin.