Recombinant Porcine IL-1β

Species

Gene ID

Accession

Source

Length

MW

Tag

Porcine

397122

P26889

E. coli

153aa

17.6kDa

－

About this Antibody

Name

Recombinant Porcine IL-1β (Recombinant Porcine Interleukin-1β; rPoIL-1β)；重组猪白细胞介素1β

Synonyms

IL1B; IL-1F2; catabolin; Catabolin; IL-1; IL-1B; IL1-BETA; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0ng/ml, corresponding to a specific activity of >2.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 3% trehalose.

Endotoxin

Less than 1EU/μg of rPoIL-1β as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant porcine interleukin-1 beta is a 17kDa protein containing 153 amino acid residues and it shares 63%-70%a.a. sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1β.

Amino Acid Sequence

ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP

产品编号

产品名称

包装

P6452-2μg

Recombinant Porcine IL-1β

2μg

P6452-10μg

Recombinant Porcine IL-1β

10μg

P6452-100μg

Recombinant Porcine IL-1β

100μg

P6452-1mg

Recombinant Porcine IL-1β

1mg

-

说明书

1份