Recombinant Protein A, His-tag

Species

Gene ID

Accession

Source

Length

MW

Tag

－

－

－

E. coli

306aa

34.7kDa

His

About this Protein

Name

Recombinant Protein A, His-tag (Recombinant Protein A, His-tag; Protein A, His-tag)；重组蛋白A，His标签

Synonyms

N/A

Purity

>97% by SDS-PAGE and HPLC analyses

Biological Activity

The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from additive free solution.

Endotoxin

Less than 0.1EU/μg of Protein A, His-tag as determined by LAL method.

Reconstitution

Dissolve in distilled water or saline.

Category

Protein A

Background

Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series.

Amino Acid Sequence

MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHH

产品编号

产品名称

包装

P5003-10mg

Recombinant Protein A, His-tag

10mg

P5003-100mg

Recombinant Protein A, His-tag

100mg

P5003-1g

Recombinant Protein A, His-tag

1g

-

说明书

1份